Assuntos
Hipertermia Maligna/etiologia , Hipertermia Maligna/terapia , Assistência Perioperatória/métodos , Canais de Cálcio Tipo L/genética , Proteínas de Ligação ao Cálcio/genética , Calsequestrina , Comorbidade , Dantroleno/uso terapêutico , Predisposição Genética para Doença , Humanos , Incidência , Hipertermia Maligna/genética , Hipertermia Maligna/mortalidade , Proteínas Mitocondriais/genética , Relaxantes Musculares Centrais/uso terapêutico , Prevalência , Canal de Liberação de Cálcio do Receptor de Rianodina/genéticaRESUMO
A conserved herpesviral kinase, designated ORF36 in murine gamma-herpesvirus 68 (MHV-68), plays multiple vital roles in the viral life cycle. Here, we show by screening mutant viruses that ORF36 counteracts the antiviral type I interferon (IFN) response. ORF36 specifically binds to the activated form of interferon regulatory factor 3 (IRF-3) in the nucleus, inhibiting IRF-3 interaction with the cotranscriptional activator CBP and thereby suppressing the recruitment of RNA polymerase II to the interferon beta promoter. The anti-IFN function of ORF36 is conserved among herpesvirus subfamilies, although the conserved kinase activity is not absolutely required for this function. MHV-68 lacking ORF36 induces a greater interferon response and is attenuated in vitro and in vivo, where acute viral infection in the lung and latency in the spleen are compromised. Our data suggest that herpesviruses have evolved within their conserved kinase an anti-IFN activity critical for evasion of host immunity and for persistence.
Assuntos
Infecções por Herpesviridae/imunologia , Fator Regulador 3 de Interferon/antagonistas & inibidores , Interferon Tipo I/biossíntese , Proteínas Quinases/metabolismo , Rhadinovirus/patogenicidade , Proteínas Virais/metabolismo , Animais , Pulmão/imunologia , Pulmão/virologia , Proteínas de Membrana/metabolismo , Camundongos , Fosfoproteínas/metabolismo , Ligação Proteica , Proteínas Quinases/genética , Rhadinovirus/genética , Baço/imunologia , Baço/virologia , Proteínas Virais/genética , Virulência , Fatores de Virulência/genética , Fatores de Virulência/metabolismoRESUMO
Gammaherpesviruses establish life-long persistency inside the host and cause various diseases during their persistent infection. However, the systemic interaction between the virus and host in vivo has not been studied in individual hosts continuously, although such information can be crucial to control the persistent infection of the gammaherpesviruses. For the noninvasive and continuous monitoring of the interaction between gammaherpesvirus and the host, a recombinant murine gammaherpesvirus 68 (MHV-68, a gammaherpesvirus 68) was constructed to express a firefly luciferase gene driven by the viral M3 promoter (M3FL). Real-time monitoring of M3FL infection revealed novel sites of viral replication, such as salivary glands, as well as acute replication in the nose and the lung and progression to the spleen. Continuous monitoring of M3FL infection in individual mice demonstrated the various kinetics of transition to different organs and local clearance, rather than systemically synchronized clearance. Moreover, in vivo spontaneous reactivation of M3FL from latency was detected after the initial clearance of acute infection and can be induced upon treatment with either a proteasome inhibitor Velcade or an immunosuppressant cyclosporine A. Taken together, our results demonstrate that the in vivo replication and reactivation of gammaherpesvirus are dynamically controlled by the locally defined interaction between the virus and the host immune system and that bioluminescence imaging can be successfully used for the real-time monitoring of this dynamic interaction of MHV-68 with its host in vivo.
